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Efek Antioksidan Kombinasi Ekstrak Etanol Acalypha indica dan Centella asiatica pada Fungsi Hati Tikus Pascahipoksia Sistemik

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Efek Antioksidan Kombinasi Ekstrak Etanol Acalypha indica dan Centella asiatica pada Fungsi Hati Tikus Pascahipoksia Sistemik

Agnes Frethernety, Melva Louisa, Novi S. Hardiany, Adisti Dwijayanti, Erni H. Purwaningsih

Abstract


Hipoksia adalah defisiensi suplai oksigen ke dalam sel atau jaringan karena gagalnya sistem respirasi yang membawa oksigen sehingga mengakibatkan kerusakan jaringan. Hati merupakan organ yang sensitif terhadap hipoksia. Tanaman Acalypha indica dan Centella asiatica memiliki efek antioksidan dan dapat melindungi banyak organ dari hipoksia. Tujuan penelitian ini menganalisis pengaruh pemberian kombinasi ekstrak etanol A.indica dan C.asiatica pascahipoksia sistemik terhadap fungsi hati, stres oksidatif dan aktivitas antioksidan hati. Sebanyak 28 tikus spraquedawley dibagi secara acak menjadi 7 kelompok. Kelompok kontrol adalah perlakuan tanpa hipoksia, perlakuan enam kelompok lainnya pascahipoksia 7 hari diberikan zat uji sebagai berikut: air, kombinasi dosis 1 dan 2, dosis tunggal A.indica, dosis tunggal C. asiatica dan dosis tunggal vitamin C selama 7 hari. Parameter yang diukur adalah aktivitas ALT dan AST, kadar MDA, rasio GSH/GSSG. Tidak ada perbedaan aktivitas ALT dan AST yang bermakna pada semua kelompok. Kadar MDA meningkat pada kelompok pascahipoksia 7 hari dibanding kontrol (p=0,007). Kelompok kombinasi 1 memiliki MDA yang rendah, rasio GSH/GSSG dan aktivitas SOD yang meningkat dibandingkan kelompok pascahipoksia 7 hari. Pemberian zat uji kombinasi 1 memiliki efek perlindungan pada hati tikus terhadap pascahipoksia 7 hari melalui mekanisme stres oksidatif dan antioksidan.

Kata kunci: Acalypha indica, Centella asiatica,hipoksia sistemik, antioksidan

 

The Effect of A. Indica  and C.Asiatica Ethanol Extract Combination on Rats Liver Function Post-Hypoxic Condition

Abstract

Hypoxia occurs due to the deficiency of oxygen supply to cells or tissue caused by the failure of the respiratory system that carries oxygen which results in cell or tissue damage. Liver is an organ sensitive to hypoxia. Acalypha  indica  and Centella asiatica are proven to have antioxidant effects and can protect many organs from hypoxic conditions. This study was aimed to analyse the effect of A. indica  and C.asiatica ethanol extract combination on post-hypoxic condition towards liver function, oxidative stress and antioxidant activity of the liver. Twenty-eight spraque-dawley rats divided randomly into 7 groups. Control group was treated without hypoxia and the other six groups were given substances as follows on the 7th day of hypoxia: water, combination of dose 1 and 2, single dose of A. indica, single dose of C.asiatica, and single dose of vitamin C for 7 days. Parameters measured were activities of ALT and AST, MDA, GSH / GSSG ratio and SOD activity. There was no significant difference in the activity of ALT and AST in all groups. MDA levels was increased in the 7-days post-hypoxic group compared to control (p=0,007). The combination 1 group had low MDA and increased GSH/GSSG ratio and SOD activity compared to the post-7days-hypoxic group. The substance of combination 1 has protective effect on the rats’ liver on post-7-days-hypoxic through oxidative stress and antioxidant mechanisms.

Keywords: Acalypha indica, Centella asiatica, systemic hypoxia, antioxidant


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