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Methods of Nucleic Acid Transfer as Basic Gene Therapy

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Methods of Nucleic Acid Transfer as Basic Gene Therapy

Novi Silvia Hardiany


The advancement of molecular biology provides benefit in the field of medicine to develop genetherapy. The aim of gene therapy is to repair the genetic damage or to replace damaged gene with thenormal gene. Delivery of gene is carried out by transfection technique, a technique to transfer nucleic acidinto eukaryote cells either using viral vectors (known as transduction), and also using non viral methodsuch as chemical substance, lipid and physical method. Some of the viral vectors used in the transductionare retrovirus, adenovirus, Adeno-associated virus (AAV) and Herpes Simplex Virus (HSV). The success oftransfection is determined by various factors which can be assessed using several reporters such as GreenFluorescence Protein (GFP).


gene therapy; non viral transfection; transduction; viral vector.

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