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Photoelectrochemical Detection of Dengue-Related Oligonucleotide Sequence using Anthocyanin as an Intercalating Agent and Electrochromic Material

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Photoelectrochemical Detection of Dengue-Related Oligonucleotide Sequence using Anthocyanin as an Intercalating Agent and Electrochromic Material

Aryo Tedjo, Anwar S. Ibrahim, Kusmardi Kusmardi

Abstract


World Health Organization (WHO) presupposes a confirmation of dengue virus infection diagnosis with two criteria, i.e. clinical and laboratory criteria. One of the basic methods used by most laboratories to diagnose dengue virus is to detect oligonucleotide sequence using a DNA amplification technique. In this research, the measurement of denguerelated oligonucleotide was conducted by photoelectrochemical method. The presence of oligonucleotide sequence in target DNA can be detected by DNA probe that is immobilized on TiO2 electrode. The DNA hybrid is then bound to electrochromic substance like anthocyanin that generates current when it is subjected to light. The photocurrent is directly proportional to the number of target DNA. The aim of this research is to obtain photoelectrochemical system that has sensitivity and high responsiveness toward the change in oligonucleotide concentration, especially the applicability of anthocyanin as a electrochromic substance and intercalating agent. Linearity (R2) generated from the change of current in response to concentration changes of target DNA (in the concentration range of 0.75–3.00 nM) is 0.9611. Thus, this method has the potential to be developed to detect the presence of dengue virus in biological sample.


Keywords


anthocyanin extract; dengue-related oligonucleotide; photoelectrochemical

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