print ISSN 2407-2354

Aktivitas Antioksidan dan Penetapan Kadar Fenol Total Ekstrak Metanol Dedak Bebeapa Varietas padi (Oryza sativa L.)

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Abstract


Antioxidant has been known to have an important role in the prevention and treatment
of diseases. In recent years, many plants have been explored to obtain new
antioxidants from natural products. Rice has been known rich in antioxidative compounds.
However, there is little attention to the co- or by- products from rice (Oryza
sativa) milling plant. One of the products is rice bran. In this study ten varieties of
rice bran, which are commonly cultivated varieties in Indonesia were studied their
antioxidant activity and total phenolic content. The antioxidant activity was examined
by DPPH scavenging assay and total reducing power, while total phenolic content
was determined by Folin-Ciocalteu reagent. The results of this study showed that
the methanolic extract of IR-64 variety demonstrated the strongest DPPH radical
scavenger activity with IC50 350.64 ppm, while in reducing power activity was exhibited
by methanolic extract of IR-42 variety. However, the highest total phenolic
content (71.85 mg gallic acid equivalent/gram) was found in methanolic extract of
OM-4495 rice bran. These result indicated that the methanolic extract of rice bran
might potentially be natural antioxidants.


Keywords : Antioxidant, DPPH scavenging assay, Folin-Ciocalteu, Oryza sativa,
rice bran, total phenolic


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