Vol 16, No 2 (2012) > Articles >

Improved Optical Probe for Measuring Phytoplankton Suspension Concentrations Based on Optical Fluoresensce and Absorption

Retno Purnamaningsih 1 , Nining Prihantini 2

Affiliations:

  1. Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
  2. Department of Biology, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok 16424, Indonesia

 

Abstract:

This paper discusses the results of improved optical probe that works according to optical fluorescence and absorption phenomena for measuring the phytoplankton suspension concentrations. Measurements are made on the Scenedesmus sp. culture. The laboratory test has shown that range of concentration from 104  up to around 1 x 106 cells/mL; fluorescence intensity at λ= 685 nm; and logarithmic of transmission intensity at λ= 405 nm are proportional to the cell concentration linear with proportional constant γ = 4 × 10-5 and β = –2 × 10-7 mL/cell respectively.

Keywords: optical probe, optical fluorescence, optical absorption, phytoplankton concentration
Published at: Vol 16, No 2 (2012) pages: 116-120
DOI:

Access Counter: 1228 views, 659 PDF downloads, .

Full PDF Download

References:

B.R. Vashishta, Botany for Degree Students: ALGAE, S. Chand, New Delhy, India, 1999, p.456.

L.E. Graham, W. Wilcox, Algae, Prentice Hall Inc., Upper Saddle River, USA, 2000, p.640.

E.J. D’Sa, S.E. Lohrenz, Applied Optic 38/12 (1999) 2524.

R.K.Y. Chan, K.M. Un, Applied Optic 40/12 (2001) 2956.

A.W. Herman, B. Beanlands, E.F. Philips, J. Plankton Res. 26 (2004) 1135.

J. Watson, S. Alexander, G. Craig, C.C. Hendry, P.R. Hobson, R.S. Lampitt, J.M. Marteau, H. Nareid, M.A. Palyer, K. Tipping, Meas. Sci. Technol. 12 (2001) L9.

E. Malkiel, O. Alquaddomi, J. Katz, Meas. Sci. Technol. 10 (1999) 1142

R.W. Novia, A. Handojo, H. Kurniawan, S. Sardy, Proceedings of Indonesia-Japan Joint Scientific Symposium, Chiba, Japan, 2004.

R. Wigajatri, A. Handojo, H. Kurniawan, N.B. Prihantini, Proceeding of The 8th International Conference on Quality in Research, Department of Electrical Engineering, University of Indonesia, Depok, Indonesia, 2005.

R. Wigajatri, N.B. Prihantini, A. Udhiarto, Prosiding Seminar Nasional Aplikasi Fotonika, Kampus ITS, Surabaya, 2008.

R.W.P. Novia, N.B. Prihantini, A. Udhiarto, Proceedings of Indonesia-Japan Joint Scientific Symposium, University of Chiba, Chiba, Japan, 2008.

R. Wigajatri, A. Handojo, H. Kurniawan, N.B. Prihantini, M.R.T. Siregar, Proceeding the International Conference on Opto-Electronics and Laser Application, Faculty of Engineering, University of Indonesia, Jakarta, Indonesia, 2002.

R. Wigajatri, N.B. Prihantini, A. Udhiarto, Instrumentasi 34/2 (2010) 137.

R. Wigajatri, N.B. Prihantini, RUUI Research Report, Universitas Indonesia, 2007.

W.D.P. Stewart, Algal Physiology and Biochemistry, Blackwell Scientific Publications

Ltd., California, 1974, p.346.

J.R.J.D. Ingle, S.R. Crouch, Spectrochemical Analysis, Prentice Hall, New Jersey, 1988, p.494.

R.W. Purnamaningsih, Doctor Desertation, Graduate Study Program on Opto-Electrotechnique and Laser Application, Faculty of Engineering, University of Indonesia, Jakarta, Indonesia, 2006.