Vol 17, No 1 (2013) > Articles >

Biological Pretreatment of Oil Palm Frond Fiber Using White-Rot Fungi for Enzymatic Saccharification

Euis Hermiati 1 , Sita Anita 1 , Lucky Risanto 1 , Dyah Styarini 2 , Yanni Sudiyani 2 , Achmad Hanafi 2 , Haznan Abimanyu 2

Affiliations:

  1. R&D Unit for Biomaterials, Indonesian Institute of Sciences, Cibinong, Bogor 16911, Indonesia
  2. Research Center for Chemistry, Indonesian Institute of Sciences, Puspiptek, Serpong, Tangerang 15340, Indonesia

 

Abstract: Oil palm frond is one type of lignocellulosic biomass abundantly and daily available in Indonesia. It contains cellulose which can be converted to glucose, and further processed to produce different kinds of value –added products. The aim of this research is to study the effects of biological pretreatment of oil palm frond (OPF) fiber using Phanerochaete chrysosporium and Trametes versicolor on the enzymatic saccharification of the biomass. The OPF fiber (40-60 mesh sizes) was inoculated with cultures of the two fungi and incubated at 27 °C for 4 weeks. The samples were taken after 1, 2, 3, and 4 weeks of incubation. Chemical components of the biomass after pretreatment were analyzed. The saccharification of the pretreated samples using cellulase and β-glucosidase was performed in a water bath shaker at 50 °C for 48 hours. The concentration of reducing sugar increased with increasing of incubation time, either in those pretreated with culture of P. chrysosporium or with T. versicolor. Pretreatment of OPF fiber using single culture of T. versicolor for 4 weeks gave the highest reducing sugar yield (12.61% of dry biomass).
Keywords: enzymatic saccharification, oil palm frond, reducing sugar, white-rot fungi
Published at: Vol 17, No 1 (2013) pages: 39-43
DOI:

Access Counter: 1242 views, 911 PDF downloads, .

Full PDF Download

References:

E.D. Wong, A.K. Razali, S. Kawai, Proceedings of The 3rd International Wood Science Symposium, Uji, Kyoto, Japan, 2000, p.153.

B. Khamseekhiew, J.B. Liang, Z.A. Zelan, C.C. Wong, Songklanakarin J. Sci. Technol. 24 (2002) 209.

W.D. Wan Rosli, K.N. Law, Z. Zainuddin, R. Asro, Bioresour. Technol. 93 (2004) 233.

Erwan, M.R. Ismail, H.M. Saud, S.H. Habib, S. Siddiquee, H. Kausar, African J. Microbiol. Res. 6 (2012) 4084.

M.A.K.M. Zahari, M.R. Zakaria, H. Ariffin, M.N. Mokhtar, J. Salihon, Y. Shirai, M.A. Hasan, Bioresour. Technol. 110 (2012) 566.

Syafwina, E.D. Wong, Y. Honda, T. Watanabe, M. Kuwahara, Proceedings of the 4th International Wood Science Symposium, Serpong, Indonesia, 2002, p.351.

C.S. Goh, K.T. Lee, S. Bhatia, Bioresour. Technol. 101 (2010) 7362.

C.S. Goh, H.T. Tan, K.T. Lee, Bioresour. Technol. 110 (2012) 662.

L.S. Hong, D. Ibrahim, I.C. Omar, The Internet J. Bioeng. 4 (2010) 6. DOI: 10.5580/1f4e.

P. Namoolnoy, S. Phoolphundh, A. Wongwicharn, Kasetsart J. (Nat. Sci.) 45 (2011) 254.

N. Moredo, M. Lorenzo, A. Dominguez, D. Moldes, C. Cameselle, A. Sanroman, World J. Microbiol. Biotechnol. 19 (2003) 665.

Anon., Mokushitsu Kagaku Jiken Manual, Japan Wood Research Society Publisher, Tokyo, 2000.

M. Selig, N. Weiss, Y. Ji, Laboratory Analytical Procedure (LAP), Technical Report NREL/TP-510-42629, National Renewable Energy Laboratory, Golden, Colorado, 2008.

R.E. Wrolstad, T.E. Acree, E.A. Decker, M.H. Penner, D.S. Reid, S.J. Scwartz, C.F. Shoemaker, D. Smith, P. Sporns (Eds.), Handbook of Food

Analytical Chemistry: Water, Proteins, Enzymes, Lipids, and Carbohydrates, John Wiley & Sons, Hoboken, New Jersey, 2005, p.655.

R.A. Ermawar, D.H.Y. Yanto, Fitria, E. Hermiati, Widya Riset 9 (2006) 197 (In Indonesian).

S.H. Anita, T. Fajriutami, Fitria, R.A. Ermawar, D.H.Y. Yanto, E. Hermiati, Teknologi Indonesia 34 (2011) 33 (In Indonesian).