Vol 18, No 1 (2014) > Chemical Engineering >

Bioethanol Production from Iles-Iles and Sorghum Starch as Raw Materials (Effect of CaCl 2 Addition and Saccharification Time)

Kusmiyati Kusmiyati 1 , Ali Mahmudi 2


  1. Renewable Energy Research Centre, Muhammadiyah University of Surakarta, Surakarta 57102, Indonesia
  2. Engineering Faculty, Chemical Engineering Department, Muhammadiyah University of Surakarta, Surakarta 57172, Indonesia


Abstract: Bioethanol produced from agricultural sources is one alternative energy that has been developed to substitute for petroleum. However, the use of food sources such ascorn and cassava to produce bioethanol still face obstacles that lead to the scarcity of food and an increase in food prices. The aim of this study was to produce bioethanol from sorghum and iles-iles, which are not used by humansas food, especially in Indonesia. For both materials, the variables studied were saccharification time (4, 8, 24, and 48 h) and concentration of CaCl2added to the liquefaction slurry (0, 100, 200, 300, and 400 mg/L). The bioethanol was produced by enzymatic hydrolysis, which consists of liquefaction usingα-amylase at 1.6% v/w (t = 1 h; T = 95-100 °C; pH 6) and saccharification of the liquefaction slurry by using β-amylase at 3.2% v/w (t = various; T = 60 °C; pH 5) and fermentation by Saccharomyces cerevisiae(t = 120 hours; pH 4, 5; yeast 5g). The best conditions were obtained for sorghum at saccharification t = 24 h, which resulted in the highest ethanol yield, 91.5 g/L, and for iles-iles at t = 48 h, which yielded 107.1 g/L. The optimum amount ofCaCl2to be added was 200mg/L, which resulted in the highest ethanol yield, 98.5 g/L and 92.4 g/L for sorghum and iles-iles, respectively. This results shows that sorghum and iles-iles are promising raw materials for the production of bioethanol, since they produce it in large amounts. 
Keywords: bioethanol, CaCl2, iles-iles, S. cerevisiae, sorghum
Published at: Vol 18, No 1 (2014) pages: 29-35

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