Vol 21, No 1 (2017) > Mini Conference >

In Vitro Toxicity Evaluation of Caffeine Imprinted Polymer (CAF-MIP) for Decaffeination Method on Normal Chang Liver Cells

Fatimah Hashim 1 , Faizatul Shimal Mehamod 2 , Naizatul Akmal Nawi 2


  1. School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
  2. Pusat Pengajian Sains Asas, Universiti Malaysia Terengganu 21030 Kuala Terengganu, Terengganu Malaysia


Abstract: Over consuming of caffeine is one of the factors to a few health problems such as insomnia, hypertension and cardiovascular disease. This preliminary study was conducted to evaluate the Caffeine-Imprinted Polymer (CAF-MIP) toxicity that was synthesized for a new alternative method for decaffeination. It is crucial to evaluate the toxicity of CAF-MIP as this product is potential to be used as complimentary with any drinks containing caffeine. In this study, the CAF-MIP toxicity potential was confirmed on Normal Chang Liver cell (NCLC) based on its IC50 value and acridine orange and propidium iodide (AO/PI) staining for mode of cell death observation. Proliferation assay was also conducted after 24, 48 and 72 hours at 30 µg/ml on NCLC and it showed that CAF-MIP promote NCLC growth as shown by at various concentration of CAF-MIP increase the percentage of NCLC viability. Observation under light microscopes on NCLC incubated wit CAF-MIP and NIP showed the normal, viable cell morphology, cuboidal and monolayer cell morphology and this can be seen with green fluorescence when view under fluorescence microscope. In conclusion, from this study, it is proved that the CAF-MIP does not initiate toxicity effects on human liver cells, meanwhile induction of cell proliferation was observed.
Keywords: caffeine, toxicity, in vitro, liver cells, cytotoxicity
Published at: Vol 21, No 1 (2017) pages: 19-25

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B. Mariam, R.J. Carnachan, N.R. Cameron, S.A. Przyborski, J. Anatomy, 211/4 (2007) 567.

T. Mossman, J. Immunol. Methods, 65 (1983) 55

S.A. Chintalwar, B. Rajkapoor, P.D. Ghode, Int. J. Pharma. Bio. Sci. 3 (2012)155.

J.M. Sargent, C.G. Taylor, Br. J. Cancer, 60 (1989) 206.

Culture of Animal Cells-Basic Techniques, Roche Diagnostics GmbH, Mannheim, Germany, 2012, p.16.

S.S. Saini, A. Kaur, Minireview: Adv. Nanopart. 2 (2013) 60.

N. Takuwa, Y. Fukui, Y. Takuwa, Mol. Cell. Biol. 19/2 (1999) 1346.

B. Ekwall, V. Silano, A. Paganuzzi-stammati, F. Zucco, Toxicity Tests with Mammalian Cell Cultures. Short-term Toxicity Tests for Non-genotoxic Effects. John Wiley & Sons Ltd., New Jersey,

USA, 1990, p.345.

A. Zimmermann, Med. Sci. Monit. 8 (2002) 53.

A.H. Moraco, H. Kornfeld, Semin. Immunol. 26/6 (2014) 497. http://dx.doi.org/10.1016/j.smim.

G. Vasapollo, R.D. Sole, L. Mergola, M.R. Lazzoi, A. Scardino, S. Scorrano, G. Mele, Int. J. Molecular Sci. 12 (2011) 5908.

A. Zunino, P. Degan, T. Vigo, A. Abbondandolo, Mutagenesis, 16 (2001) 28

K. Mascotti, J. McCullough, S.R. Burger, Transfussion, 40 (2000) 693.

G.J. Troup, D.R. Hutton, J. Dobbie, J.R. Pilbrow, C.R. Hunter, B.R. Smith, B.J. Bryant, Med. J. Australia, 148/10 (1988) 537.

J.K. Willcox, S.L. Ash, G.L. Catignani, Crit. Rev. Food Sci. Nutr., 44/4 (2004) 275.

C. Cerella, S. Coppola, V. Maresca, M. De Nicola, F. Radogna, L. Ghibelli, Ann. N.Y. Acad. Sci. 1171 (2009) 559.

H. Fatimah, A. Abdul-Manaf, M.A. Nakisah, Malays. J. Microsc. 9 (2013) 133.

A.I. Baba, Sci. Med. Vet. 12 (2009) 1.

K.M. Chan, N.K. Rajab, M.H.A. Ishak, A.M. Ali, K. Yusoff, L.B. Din, S.H. Inayat-Hussain, Chemico-Biol. Interact. 159 (2006) 129.