Vol 7, No 2 (2003) > Articles >

The Study on Fluorescence Characteristics of Chlorella spp: pH Influence on Culture

Retno Wigajatri Purnamaningsih 1 , Andrianto Handojo 2 , Hendrik Kurniawan 1 , NB Prihantini 3


  1. Program Studi Optoelektroteknika dan Aplikasi Laser, Fakultas Teknik, Universitas Indonesia, Jakarta 10430, Indonesia
  2. Departemen Teknik Fisika, Fakultas Teknologi Industri, Institut Teknologi Bandung, Bandung 40132, Indonesia
  3. Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Indonesia, Depok 16424, Indonesia


Abstract: Experiments for measuring the fluorescence characteristics of Chlorella spp. by using high stability and high repetition rate nitrogen laser of energy 5 mJ with pulse duration of 5 ns have been carried out. The results show that for a cell concentration range from 2,625 cells/ml up to 2,769,000 cells/ml, the fluorescence intensities at pada λ = 687 nm have a linear relationship with the cell concentration. It has been also found that for a 7 days old chlorella culture, the pH variation at the starting culture influenced the cell concentration.
Keywords: Chlorella spp., culture, fluorescence, pH, high stability nitrogen laser
Published at: Vol 7, No 2 (2003) pages: 83-88

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