Vol 18, No 2 (2014) > Chemical Engineering >

Digestibility of Betung Bamboo Fiber Following Fungal Pretreatment

Widya Fatriasari 1 , Wasrin Syafii 2 , Nyoman Jaya Wistara 2 , Khaswar Syamsu 3 , Bambang Prasetya 4

Affiliations:

  1. Research Center for Biomaterials, Indonesian Institute of Sciences, Cibinong, Bogor 16911, Indonesia
  2. Department of Forest Product Technology, Facultyof Forestry, Institut Pertanian Bogor, Bogor 16680, Indonesia
  3. Department of Agroindustrial Technology, Facultyof Agricultural Engineering and Technology, Institut Pertanian Bogor, Bogor 6680, Indonesia
  4. National Standardization Agency, Manggala Wanabakti Building, Senayan, Jakarta 10270, Indonesia

 

Abstract: This research evaluated the effect of fungal pretreatment of betung bamboo fibers and enzymatic- and microwave-assisted hydrolysis on the reducing sugar yield. The enzymatic hydrolysis of the pretreated biomass was carried out with cellulase and 10 and 20 FPU/g of substrate in a shaking incubator at 50 °C and 150 rpm for 48 h. The sulfuric acid concentration used in the microwave-assisted acid hydrolysis was 1.0, 2.5, and 5%, either with or without the addition of activated carbon. Microwave irradiation (330 Watt) was applied for 5–12.5 min. The yield of reducing sugar was better with the microwave-assisted acid hydrolysis, and the yield tended to increase with an increase in the irradiation time. Based on the dry weight of the initial biomass (bamboo), pretreatment with 5% inoculum loading resulted in a higher reducing sugar yield (17.06%) than with 10% inoculum loading (14.54%). At a 1% acid concentration, the formation of brown compounds decreased, followed by a reduction in the reducing sugar yield. The addition of activated carbon at a 1% acid concentration seemed to be of no benefit with respect to the yield in the microwave-assisted acid hydrolysis. The pretreatment with the 5% inoculum loading for 12.5 min at 1% acid concentration resulted in the highest reducing sugar yield. Under these conditions, the yield was 6.3-fold that of the reducing sugar yield using 20 FPU/g of cellulase. The rate of bamboo hollocellulose hydrolysis reached 22.75% of the maximum theoretical reducing sugar reducing sugar of dry biomass.
Keywords: activated carbon, betung bamboo, microwave and enzymatic hydrolysis, reducing sugar yield, white rot fungi
Published at: Vol 18, No 2 (2014) pages: 51-58
DOI:

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