Vol 23, No 2 (2019) > Chemical Engineering >

Component Analysis and Antiangiogenic Activity of Thailand Stingless Bee Propolis

Eriko Ishizu 1 , Sari Honda 1 , Tosihro Ohta 1 , Boonyadist Vongsak 2 , Shigenori Kumazawa 1

Affiliations:

  1. Department of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 424-0414, Japan
  2. Faculty of Pharmaceutical Sciences, Burapha University, Chon Buri 22170, Thailand

 

Abstract: Propolis is a resinous mixture of substances collected by honey bees from certain plants. It has gained popularity as a food and alternative medicine. We recently obtained Thailand stingless bee propolis. However, to the best of our knowledge, there are only a few studies on native Thailand stingless bee propolis. Therefore, the information on its chemical composition and biological activities is needed to investigate the potential utility of the propolis. In this study, we performed the component analysis, the functional evaluation and identification of plant origin of Thailand stingless bee propolis. Nine xanthones including α-mangostin, garcinone C, γ-mangostin, garcinone D, β-mangostin, gartanin, 8-deoxygartanin, 9-hydroxycalabaxanthone, and mangostanol were isolated from propolis. Comparative analysis of the 70% ethanol extracts of Thailand stingless bee propolis (EEP) and the yellow resin from the fruit surface of Garcinia mangostana (EEM) was performed using reversed-phase high-performance liquid chromatography coupled with high resolution-electrospray mass spectrometry. The extracts showed the similar chromatographic patterns. This result suggests that the plant origin of Thailand stingless bee propolis is the yellow resin from fruit surface of G. mangostana. Furthermore, EEP and EEM were tested for their ability to inhibit the tube formation of human umbilical vein endothelial cells (HUVECs).
Keywords: Thailand stingless bee propolis, plant origin, Garcinia mangostana, angiogenesis, tube formation
Published at: Vol 23, No 2 (2019) pages: 77-82
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