Vol 24, No 2 (2020) > Chemical Engineering >

Evaluation of IL-17A and IL-17F Gene Expression in Peripheral Blood Mononuclear Cells in Different Clinical Stages of Chronic Hepatitis B Infection in an Iranian Population

Tannaz Akbari Kolagar 1 , Seyed Reza Mohebbi 2 , Fatemeh Ashrafi 1 , Shahrzad Shoraka 3 , Hamid Asadzadeh Aghdaei 3 , Mohammad Reza Zali 2

Affiliations:

  1. Faculty of Biological Sciences, Islamic Azad University, Tehran-North Branch, Tehran 1651153311, Iran
  2. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran
  3. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran

 

Abstract: Hepatitis B virus (HBV) infection is one of the main causes of liver damage, which can also lead to chronic hepatitis B (CHB) infection. More than 240 million individuals worldwide are chronic carriers of HBV. Among individuals with CHB who are untreated, approximately 15% – 40% will progress to liver cirrhosis or cancer. The interactions between HBV and host immune response play significant roles in the progression of CHB. CHB can be generally divided into four different clinical phases: immune tolerance (IT), immune clearance, inactive carrier, and Hepatitis B surface antigen (HBsAg)-negative reactivation phase (ENEG). Many studies showed that interleukins play important roles in anti-viral immunity and pathogenesis of chronic hepatitis. However, the relations between clinical phases of CHB and host immune transcriptome remain unclear. In this study, we aimed to investigate the expression of interleukin-17A (IL-17A) and IL-17F genes in the peripheral blood mononuclear cells (PBMCs) of patients with CHB through different clinical stages. Results were compared with the control group, which comprised individuals with no history of pre-existing medical conditions. This case–control study was carried out on 32 patients with CHB as the case group and 32 healthy individuals as the control group. According to clinical data, CHB cases were divided into two groups: active (n = 22) and inactive (n = 11). PBMC samples were obtained from all groups. After total RNA extraction and cDNA synthesis, real-time PCR was used to determine IL-17A and IL-17F expression levels. The results were analyzed by REST software, SPSS, and GraphPad Prism. The IL-17A and IL-17F gene expression levels were observed to be significantly higher in the CHB group than in the control group (IL-17A: P = 0.0013; IL-17F: P = 0.0103). The active phase group (including IT, clearance, and reactivation samples) significantly increased in comparison with the inactive phase (IL-17A: P = 0.000; IL-17F: P = 0.000). The study suggests that IL-17A and IL-17F do not only activate inflammation but are also involved in HBV-related disease progression and chronicity. Thus, mRNA levels of IL-17A and IL-17F could be used as a biomarker to diagnose CHB infection and distinguish between the active CHB phase from the inactive phase.
Keywords: bandpass filter, microstrip, optimization, resonator, ultra wideband
Published at: Vol 24, No 2 (2020) pages: 59-64
DOI:

Access Counter: 122 views, 77 PDF downloads, .

Full PDF Download

References:

C. Boni, P. Fisicaro, C. Valdatta, B. Amadei, P. Di

Vincenz o, T. Giubert i, et al . J . Viro l 81 8 (2007 4215.

W.M. Lee. N Engl J. Med. 337 24 (1997 1733.

Liver EAFTSOT. J . Hepatol 67 2 (2017 370.

D. Lavanchy, M. Kane. Hepatitis B Virus in

Human Diseases, Springer 2016 p. 187.

D. Ganem, A.M. Prince. N Engl J Med. 350 11

) 1118.

R. Jamali, N. E Daryani. KAUMS J ( 13 4

(2010 332.

L.S. Tang, E. Covert, E. Wilson, S. Kottilil Jama.

17 (2018 1802.

H. Zhang, F. Bernuzzi, A. Lleo, X. Ma, P.

Invernizzi. Me diat . Inflamm . 2015 2015 1

M. F. Yuen, S.H. Ahn, D. S. Chen, P. J. Chen,

G.M. Dusheiko, J. L. Hou, et al . J . Clin.

Gastroenterol. 50 4 (2016 286.

Y. Yang, J. Dai, M. Yan, M. Yue, X.H. Wang, X.

Min, et al . Eur J Inflamm . 16 (2018)

X. E. Xi, Y. Liu, Y. Lu, L. Huang, X. Qin, S. Li

Med Oncol. 32 1 (2015 355.

J. Wang, Y. Liu, L. Xie, S. Li, X, Qin Clin Res.

Hepatolo Gastroenterol. 40 3 (2016 288.

J.C. Waite, D, Skokos Int . J. Inflamm . 2012 2012

H. Spits, J.H. Bernink, L. Lanier. Nature Immunol

7 (2016 758.

W. K. Seto, Y. R. Lo, J. M. Pawlotsky, M. F. Yuen

Lancet. 392 10161 (2018 2313.

S. Romani, S. Hosseini, S. Mohebbi, A. Boonstra,

A. Sharifian. J . Viral Hepatitis. 24 9 (2017 776.

H. Park, Z. Li, X.O. Yang, S.H . Chang, R. Nurieva,

Y. H. Wang, et al . Nature Immunol. 6 11 (2005

M. Oukka. Ann. Rheum. Dis. 67 Suppl 3 (2008

iii26.

S.H. Mohaghegh, P. Karayiannis, S. Ashtari, M.A.

Mahmanzar, B. Khanabadi, N. Modami, et al .

Demographic Changes of Hepatitis B Virus

Infection in Iran for the Last Two Decades 2017.

D. S. Ming, Z. Z. Lin, X. D. Qiu. Int J . Clin Exp

Med 9 6 (2016 9437.

Z. M Jilkova, S. Afzal, H. Marche, T. Decaens, N.

Sturm, E. Jouvin Marche, et al . Liver 36 8

(2016 1116.

F. Lafdil, A.M . Miller, S.H. Ki, B, Gao Cell . Mol.

Immunol. 7 4 (2010 250.

J.K. Kolls, A, Lindén Immun. 21 4 (2004 467.

M. Khalili, M.C. Shuhart, M. Lombardero, J.J.

Feld, D.E. Kleiner, R.T. Chung, et al . Diabetes

Care. 41 6 (2018 1251.

C.A. Hunter, S.A. Jones. Nat Immunol 16 5

(2015 448.

F. Q. Hou, L. W. Song, Q. Yuan, L. L. Fang, S. X.

Ge, J. Zhang, et al . 5 3 (2015 218.

C. Gu, L. Wu, X. Li Cytokine. 64 2 (2013 477.

C. J. Chen, H. I. Yang, J. Su, C. L. Jen, S. L. You,

S. N. Lu, et al . 295 1 (2006 65.

E. Bettelli, T. Korn, M. Oukka, V.K. Kuchroo

Nature. 453 7198 (2008 1051.

A. Beringer, M. Noack, P. Miossec. Trends Mol.

Med 22 3 (2016 230.

B. Becher, S. Spath, J. Goverman. Nature Rev

Immunol. 17 1 (2017 49.

S. Bao, J. Zheng, G. Shi. Mol Med. Rep. 6 4

(2017 3713.

Y. Q. Wang, W. J. Cao, Y. F. Gao, J. Ye, G. Z. Zou.

World J. Gastroenterol. 24 12 (2018 1312.

B.T. Sy, N.X. Hoan, H. Van Tong, C.G. Meyer,

N.L. Toan, L.H. Song, et al . World J.

Gastroenterol. 24 2 (2018 248.

M. Nomura, M. Tsuge, T. Uchida, N. Hiraga, M.

Kurihara, K. Tsushima, et al . J . Viral Hepat 25 12

(2018 1555.

Z. Ma, Q. Cao, Y. Xiong, E. Zhang, M. Lu

Vaccines. 6 1 (2018 6.

K. Stevens. Hepatitis B Infection: Immune

Mechanisms Underlying Acute Infection: Johns

Hopkin s University 2018.

X. Li, X. Liu, L. Tian, Y. Chen. Clin Rev. Allergy

Immunol 50 1 (2016 41.

A. Askari, R. Nosratabadi, M. Khaleghinia, N.

Zainodini, D. Kennedy, Z. Shabani, et al . Viral

Immunol 29 9 (2016 496.

S.B. Wiegand, B. Beggel, A. Wranke, E. Aliabadi,

J. Jaroszewicz, C. J. Xu, et al . Sci Rep . 9 1 (2019 1.

M. Świderska, J. Jaroszewicz, A. Parfieniuk

Kowerda, M. Rogalska Płońska, A. Stawicka, A.

Panasiuk, et al . Mediat . Inflamm. 2017 2017 1

L. Monin, S.L. Gaffen. Cold Spring Harbor

Perspect. Biol 10 4 (2018 a028522.

T. Korn, E. Bettelli, M. Oukka, V.K. Kuchroo Ann .

Rev. Immunol. 27 (2009) 485.

J.Y. Zhang, Z. Zhang, F. Lin, Z.S. Zou, R.N. Xu, L.

Jin, et al . 51 1 (2010 81.

W. J. Du, J. H. Zhen, Z. Q. Zeng, Z. M. Zheng, Y.

Xu, L. Y. Qin, et al . Diagn Pathol 8 1 (2013 40.

K. Lan, S.C. Verma, M. Murakami, B. Bajaj, E.S.

Robertson. Current Protocols Microbiol 6 1 (2007

A. 4C. 1.

T. Ishimaru, K. Wada, H.T.X. Hoang, A.T.M. Bui,

H.D. Nguyen, H. Le, et al . Environ Health Prev.

Med. 22 1 (2017 9.

J.E. Song. Ann . Transl. Med. 4 18 (2016 338

S. Mohebbi, S. Amini Bavil Olyaee, N. Zali, B.

Noorinayer, F. Derakhshan, M. Chiani, et al . Clin

Microbiol. Infect. 14 9 (2008 858.

S. Mohebbi, S. Amini Bavil Olyaee, N. Zali, B.

Damavand, P. Azimzadeh, F. Derakhshan, et al . J

Med. Virol 84 3 (2012) 414.

S.M.E. Tahaei, S.R. Mohebbi, S.R. Fatemi, P.

Azimzadeh, D. Mirsattari, A. Sanati, et al .

Gastroenterol . Hepatol. 5 3 (2012) 161.

S. Horaka , S.R. Mohebbi, S.M. Hosseini, A.H.

Razavi, Y. Hatami, A. Sharifian, et al . Microb

Pathog. 128 (2019) 263.

K. Matsuura, Y. Tanaka. J Med. Virol. 88 2 (2016)

Z Q Zou, S. Zhang, Q. Lin, R. L. Qu, Y. F. Li, F.

H. Zhang, et al . I Microb Pathog. 105 (2017) 100.

L. I. Cringus, F. Petrescu V. Calborean L.T.

Rotaru, C. Marginean, C. Mesina, et al . Rev. Chim.

2 (2019) 646.

M.J. Mc Geachy , D. Cua S.L. Gaffen Immun .

4 (2019) 892.

H . Tayefinasrabadi S. Mohebbi, P. Azimzadeh B.

Hatami S. Kazemian M. S Niasar et al . J Babol

Univ Med Sci 20 1 (2018) 42.

W. Ren, Z. Wu Z, R. Ma R, Z. Liu Z, Y. Wang Y,

Wu L, et al . Genet Test. Mol. Biomark 21 5

(2017) 286.