Vol 15, No 1 (2011) > Articles >

The Synergy of Recombinant Xylanolytic Enzyme on Xylan Hydrolysis

One Asmarani 1 , Bambang Wardojo 2 , Ni Nyoman Puspaningsih 3

Affiliations:

  1. Proteomic Laboratory, Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia
  2. Department of Pharmacochemistry and Pharmacognosy, Faculty of Pharmacy, Airlangga University, Surabaya 60115, Indonesia
  3. Department of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya 60115, Indonesia

 

Abstract: Microbial xylanases or xylanolytic enzyme have received considerable attention over the last years owing to a multitude of  possible applications. These  enzymes  have  potential  in  the  biodegradation of lignocellulosic biomass  to  fuels, chemicals, fruit juice, animal feed and in improving rumen digestion. More recently, the use of xylanases as bleaching agent  in  the  pulp  and  paper  industry  has  been  suggested  to  replace  of some  of  the  chemicals  presently  used  for  this purpose. Such applications could  have an important positive impact on the environment. The purpose of  this research was  determining  the  synergy  of  3 recombinant  xylanolytic  enzymes  (β-xylosidase,  exo-xylanase  and α-L-arabinofuranosidase)  from  recombinant  Eschericia  coli  BL21 (DE-star)  in  xylan  hydrolysis  by  analysis  the  reduction sugar product. Purified of recombinant xylanolytic enzyme β-xylosidase  (Xyl), exo-xylanase  (Exo-Xyl) and α-L-arabinofuranosidase  (Abfa)  with  Ni-NTA resin.  Seven  samples  of  enzyme  (each  and  enzyme  mixture)  used to hydrolyze  xylan    substrate  (oat-spelt  xylan).  Analysis  of  hydrolysis product  was  done  by  HPLC.  The  xylanolytic activities of this enzyme before and after purification were 0,91 and 9,94 U/mL (Exo-Xyl); 1,65 and 14,2 U/mL (Xyl); 0,65  and  5,6  U/mL  (Abfa).  The  xylosidase  activity  were  2,37  and  14,3  U/mL  (Xyl);  1,49  and  10,5  U/mL  (Exo-Xyl); 2,54  and  18,6  U/mL  (Abfa).  The  highest  hydrolysis  product  of  xylan  (xylose)  shown  in  enzyme  mixture  of    exo-xylanase and β-xylosidase was 1,084 mg/mL.
Keywords: α-L-arabinofuranosidase, β-xylosidase, exo-xylanase, xylan, xylose
Published at: Vol 15, No 1 (2011) pages: 82-88
DOI:

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